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Lab Jobs

Solutions Schedule

Date
Lab Member
12/2/2019
Heenam, Chun-Hao
12/5/2019 Katie, Sandy
12/9/2019
Sarah C., Stephanie
12/12/2019 Han, Mengyi
12/16/2019 Carmie, Mark
12/19/2019 James, Wilber
1/6/2020
Sharan, Cynthia
1/9/2020
Wilber, Stephanie
1/13/2020 Wen, Yan
1/16/2020
Mengyi, Chun-Hao
1/21/2020
Wilber, Stephanie
1/23/2020 Hillel, Carmie
1/27/2020 Porfirio, Sarah C
1/30/2020
Wilber, Stephanie
2/3/2020 Katie, Heenam
2/6/2020 Sandy, Mark
2/10/2020
Wilber, Stephanie
2/13/2020 James, Cynthia
2/18/2020 Sharan, Wen
2/20/2020
Wilber, Stephanie
2/24/2020 Yan, Mengyi
2/27/2020 Chun-Hao, Hillel
3/2/2020
Wilber, Stephanie
3/5/2020 Carmie, Porfirio
3/9/2020 Sarah C., Katie
3/12/2020
Wilber, Stephanie
3/16/2020 Heenam, Sandy
3/19/2020 Mark, James
3/23/2020
Wilber, Stephanie
3/26/2020 Cynthia, Wen
3/30/2020 Sharan, Menyi
4/6/2020
Wilber, Stephanie
4/9/2020 Yan, Chun-Hao
4/13/2020 Hillel, Carmie
4/16/2020
Wilber, Stephanie
4/20/2020 Porfirio, Sarah C
4/23/2020 Katie, Heenam
4/27/2020
Wilber, Stephanie
4/30/2020 Mark, Sandy

Cold Room Cleaning Schedule

Date Lab Member
12/15/19 Cynthia, Stephanie
1/15/20 Menygi, Heenam
2/15/20 Wen, Sandy
3/15/20 Katie, Wilber
4/15/20 Porfirio, James
5/15/20 Hille, Mark
6/15/20 Chun-Hao, Stephanie
7/15/20 Cynthia, Yan
8/15/20 Mark, Sandy
9/15/20 Sharan, Heenam
10/15/20 Sarah C., Carmie
11/15/20 Stephanie, Mengyi
12/15/20 James, Wilber

Chemical Room Cleaning Schedule

Date Lab Member
12/15/19 Katie, Wilber
1/15/20 Porfirio, Hillel
2/15/20 James, Stephanie
3/15/20 Yan, Wilber
4/15/20 Wen, Chun-Hao
5/15/20 Katie, Heenam
6/15/20 Mengyi, Sandy
7/15/20 Sharan, Carmie
8/15/20 Wilber, Yan
9/15/20 Cynthia, James
10/15/20 Hillel, Katie

11/15/20

Sarah C., Mark
12/15/20 Sharan, Carmie

 

Lab Jobs

Equipment

Item

Description

Lab Member

20° Room

Maintain and organize.

Mark

Cold Room

Clean cold room monthly.  The schedule can be found here.

All Lab Members

Chemical Room

Clean chemical room monthly.  The schedule can be found here.

All Lab Members

Water Bath, K209

Maintain water level, replace water when dirty, call in repairs.

Liz

Water Bath, K219

Maintain water level, replace water when dirty, call in repairs.

Porfirio

Incubators (all Temps)

Maintain, call in repairs.  More information regarding maintaining the incubators can be found here.

Hillel

Centrifuges, Sorvall, K220

A protocol for using the Sorvall can be found here.

Heenam

4° Centrifuge, K209

Maintain, call in repairs.

Wen

Table Top Centrifuge, K217

This document has information for routine maintenance.  Information about calling in repairs can be found here.

Han

Table Top Centrifuge, K219

This document has information for routine maintenance.  Information about calling in repairs can be found here.

Porfirio

Table Top Centrifuge, K224

This document has information for routine maintenance.  Information about calling in repairs can be found here.

Carmie

PCR Machine, K209

Instruct users, maintain, call in repairs.

Wen

PCR Machine, BioRad T100, K217

Instruct users, maintain, call in repairs.  The manual for the machine can be found here.

Han

PCR Machine, BioRad C1000 Touch Thermal Cycler, K217

Instruct users, maintain, call in repairs.  The manual for the machine can be found here.

Mengyi

PCR Machine, MJ Research PTC200, K220

Instruct users, maintain, call in repairs.  The manual for the machine can be found here.

Stephanie

PCR Machine, K224

Instruct users, maintain, call in repairs.

Carmie

Electroporator, K217

Instruct users, maintain, call in repairs.  For information on how to use the Electroprorator, click here.

Porfirio

Shakers

Maintain by cleaning with bleach or alcohol, call in repairs.

Stephanie

pH Meter

Information regarding the pH meter can be found here.

Wilber

Balances

For instruction on the balances in the Sternberg lab, click here.

Wilber

DNA Gel Boxes and Power Supplies

Maintain supply of combs, call in repairs.

Sandy and Heenam

Western Blot Apparatus

Maintain, call in repairs.  The protocol for using the Western Blot Apparatus can be found here.  The instruction manual can be found here.

Wen

Lyophilizer, Alles 291

The protocol for the Lyophilizer can be found here, and if you need additional information, the user manual for the Lyophilizer can be found here.

Sarah C.

Microscopes

Item

Description

Lab Member

Camera, Worm Tracker, Light Sources

Order bulbs, clean & maintain.  For info regarding maintenance of the Worm Tracker, click here.

Sandy

Leica XCite X120 Microscope, GFP Dissecting, K221

Maintain supply of bulbs, maintain,
instruct users.  The microscope user guide can be found here, and a trouble shooting guide can be found here.  Additional info can be found here.  Additional guidelines for maintenance of the X-Cite 120 Series light source can be found here.

Cynthia and Han

Microscopes, Compound, K221

Maintain, call in repairs.

Cynthia

Microscopes, Dissecting Stereo (all)

Changing bulbs, instruct users.

Stephanie

Laser Ablation Microscope

Maintain, call in repairs.  Instructions on how to clean and refill the dye chamber can be found here.

Hillel

Injection Scope/Needle Puller, Alles 292

Maintain supply of needles & filaments,
instruct users.  For more information regarding the Injection Scope/Needle Puller, click here.

Katie

Cell Dissection Scope, Alles 292
Dissection Needle Puller, K222

 

Sandy

Calcium Imaging Rig, K216

Maintain, instruct users.  For info on how to use the Calcium Imagaing Rig, click here.

Mengyi

DIC Microscope Camera, Computer,
Software

 

Katie

Reagents

Item

Description

Lab Member

-20° Enzymes, PCR Reagents,
Antibody, K219

Keep freezer organized. Assist with finding and ordering enzymes.  Anyone that wants an enzyme should click here.

Wilber, Liz

Chemical Management

Item

Description

Lab Member

Hood/Organics, K209

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

Hood/Organics, K217

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

Hood/Organics, K220

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

Hood, K224

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

Carcinogen/Radiation Hood, Alles 182

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

TC Hood K220

Clean, maintain, proper labeling.

Sarah C.

Hood Maintenance

Schedule hood maintenance

Sarah C.

Dark Room/UV Gel Imager

 

Shared Resource

Maintenance

Item

Description

Lab Member

Lounge/Food

 

Sarah T.

Safety Officer/Supply Safety Kit

For EMERGENCIES, call x5000.  Do not call 911.  For information regarding waste disoposal, please click here.  Lab safety information can also be found in the binder located in K219 above Sarah's desk.

Sarah C.

Other

Item

Description

Lab Member

Ordering Chemicals, Supplies, 
Plasmids, RNAi, Equipment

 

Sarah T.

Lab Website

Keep content up to date.

Sarah T. and Paul

Liquid Nitrogen/Gasses

Maintain adequate supply for weekends.  Information regarding the maintenance of our LN2 stock can be found here.

Barbara

-80° Freezers

Clean, maintain, instruct users.

Barbara and Sarah T.

X-ray Source, Kerckhoff Basement (B121)

This is a shared resource.  Information regarding the use of the equipment can be found here.

Shared Resource

Pipetman

Call in service requests for calibration and/or repairs.  Info regarding service can be found here.

Sarah T.

Strain Collection

Information about freezing worms can be found here.

Barbara

Media Solutions and Plates

 

All Lab Members

Printer

Maintain and order supplies.  Information regarding printer maintenance can be found here.

Sarah T.

Microscope Front and Back Room, K221

Organize area.

Chun-Hao