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Lab Jobs

Pouring/Solutions Schedule

Media Prep Date Pouring Date Boxing Date Solutions Date
Wen Oct. 26 Cynthia, James Oct. 26 Michael Oct. 27 Sandy, Pei Oct. 24
Sarah C.  Oct. 31 Pei, Hillel Oct. 31 Michael Nov. 1 Shayda, Katie Oct. 26
Ravi Nov. 2 Katie, Porfirio Nov. 2 Michael Nov. 3 Han, James Oct. 30
Hillel Nov. 7 Wen, Ravi Nov. 7 Michael Nov. 8 Sandy, Katie Nov. 2
Jonathan Nov. 9 James, Heenam Nov. 9 Michael Nov. 10 Porfirio, Cynthia Nov. 6
Shahla Nov. 14 Shahla, Wen Nov. 14 Michael Nov. 15 Shayda, Pei Nov. 9
Sandy Nov. 16 Pei, Mengyi Nov. 16 Michael Nov. 17 Carmie, Cynthia Nov. 13
David Nov. 21 Katie, Ravi Nov. 21 Michael Nov. 22 James, Mengyi Nov. 16
Heenam Nov. 28 David, Jonathan Nov. 28 Michael Nov. 29 Katie, Wen Nov. 20
Cynthia Nov. 30 Han, Hillel Nov. 30 Michael Dec. 1 Shahla, Porfirio Nov. 27
Shayda Dec. 5 Liz, Jonathan Dec. 5 Michael Dec. 6 Heenam, James Nov. 30
Carmie Dec. 7 Sarah C., Porfirio Dec. 7 Michael Dec. 8 Liz, Han Dec. 4
Shahla Dec. 12 Shahla, David Dec. 12 Michael Dec. 13 Hillel, Liz Dec. 7
Mengyi Dec. 14 Cynthia, Heenam Dec. 14 Michael Dec. 15 Sarah C., Han  Dec. 11
Pei Dec. 19 Carmie, Jonathan Dec. 19 Michael Dec. 20 David, Heenam Dec. 14
Katie Dec. 21

Sarah C., Liz

Dec. 21 Michael Dec. 22 Shahla, Shayda Dec. 18
Shayda Jan. 2 Hillel, Porfirio Jan. 2 Michael Jan. 3 Carmie, Jonathan Dec. 21
Han Jan. 4 James, Cynthia Jan. 4 Michael Jan. 5 Ravi, Hillel Jan. 4
Mengyi Jan. 9 Carmie, Shayda Jan. 9 Michael Jan. 10 Sarah C. , Liz Jan. 9
James Jan. 11 Sandy, Liz Jan. 11 Michael Jan. 12 David, Wen Jan. 11
Wen Jan. 16 Heenam, Mengyi Jan. 16 Michael Jan. 17 Hillel, Jonathan Jan. 15
Porfirio Jan. 18 Han, Pei Jan. 18 Michael Jan. 18 Sarah C., Carmie Jan. 18
Katie Jan. 23 David, Mengyi Jan. 23 Michael Jan. 24 Ravi, Sandy Jan. 22
Liz Jan. 25 Sandy, Han Jan. 25 Michael Jan. 26 Wen, Jonathan Jan. 25
David Jan. 30 Ravi, Shayda Jan. 30 Michael Feb. 1 Cynthia, Mengyi Jan. 29

Lab Jobs

Equipment

 

 

Item

Description

Lab Member

20° Room

Maintain and organize.

Pei

Cold Room

Clean cold room monthly.  The schedule can be found here.

All Lab Members

Chemical Room

Clean chemical room monthly.  The schedule can be found here.

All Lab Members

Water Bath, K209

Maintain water level, replace water when dirty, call in repairs.

Liz

Water Bath, K219

Maintain water level, replace water when dirty, call in repairs.

Shahla

Incubators (all Temps)

Maintain, call in repairs.  More information regarding maintaining the incubators can be found here.

Hillel

Centrifuges, Sorvall, K220

A protocol for using the Sorvall can be found here.

Heenam

4° Centrifuge, K209

Maintain, call in repairs.

Pei

Table Top Centrifuge, K217

This document has information for routine maintenance.  Information about calling in repairs can be found here.

Ravi

Table Top Centrifuge, K219

This document has information for routine maintenance.  Information about calling in repairs can be found here.

Porfirio

Table Top Centrifuge, K224

This document has information for routine maintenance.  Information about calling in repairs can be found here.

Ravi

PCR Machine, K209

Instruct users, maintain, call in repairs.

Wen

PCR Machine, BioRad T100, K217

Instruct users, maintain, call in repairs.  The manual for the machine can be found here.

Han

PCR Machine, BioRad C1000 Touch Thermal Cycler, K217

Instruct users, maintain, call in repairs.  The manual for the machine can be found here.

Shayda

PCR Machine, MJ Research PTC200, K220

Instruct users, maintain, call in repairs.  The manual for the machine can be found here.

Han

PCR Machine, K224

Instruct users, maintain, call in repairs.

Ravi

Electroporator, K217

Instruct users, maintain, call in repairs.  For information on how to use the Electroprorator, click here.

Mihoko and Porfirio

Shakers

Maintain by cleaning with bleach or alcohol, call in repairs.

Shahla

pH Meter

Information regarding the pH meter can be found here.

James

Balances

For instruction on the balances in the Sternberg lab, click here.

James

DNA Gel Boxes and Power Supplies

Maintain supply of combs, call in repairs.

Sandy and Heenam

Western Blot Apparatus

Maintain, call in repairs.  The protocol for using the Western Blot Apparatus can be found here.  The instruction manual can be found here.

Wen

Lyophilizer, Alles 291

The protocol for the Lyophilizer can be found here, and if you need additional information, the user manual for the Lyophilizer can be found here.

Heenam

Microscopes

 

 

Item

Description

Lab Member

Camera, Worm Tracker, Light Sources

Order bulbs, clean & maintain.  For info regarding maintenance of the Worm Tracker, click here.

Sandy

XCite X120 Microscope, GFP Dissecting, K221

Maintain supply of bulbs, maintain,
instruct users.  The microscope user guide can be found here, and a trouble shooting guide can be found here.

Cynthia and Han

Microscopes, Compound, K221

Maintain, call in repairs.

Cynthia and Mihoko

Microscopes, Dissecting Stereo (all)

Changing bulbs, instruct users.

David

Laser Ablation Microscope

Maintain, call in repairs.  Instructions on how to clean and refill the dye chamber can be found here.

Hillel

Injection Scope/Needle Puller, Alles 292

Maintain supply of needles & filaments,
instruct users.  For more information regarding the Injection Scope/Needle Puller, click here.

Katie

Cell Dissection Scope, Alles 292
Dissection Needle Puller, K222

 

Mihoko and Sandy

Calcium Imaging Rig, K216

Maintain, instruct users.  For info on how to use the Calcium Imagaing Rig, click here.

Ravi

DIC Microscope Camera, Computer,
Software

 

Katie

Reagents

 

 

Item

Description

Lab Member

-20° Enzymes, PCR Reagents,
Antibody, K219

Keep freezer organized. Assist with finding and ordering enzymes.  Anyone that wants an enzyme should click here.

Jonathan, Liz

Chemical Management

 

 

Item

Description

Lab Member

Hood/Organics, K209

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

Hood/Organics, K217

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

Hood/Organics, K220

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

Hood, K224

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

Carcinogen/Radiation Hood, Alles 182

Clean, maintain, instruct users, proper
labeling.  The protocol regarding this hood can be found here.

Sarah C.

TC Hood K220

Clean, maintain, proper labeling.

Sarah C.

Dark Room/UV Gel Imager

 

Carmie

Maintenance

 

 

Item

Description

Lab Member

Lounge/Food

 

Sarah T.

Safety Officer/Supply Safety Kit

For EMERGENCIES, call x5000.  Do not call 911.  For information regarding waste disoposal, please click here.  Lab safety information can also be found in the binder located in K219 above Sarah's desk.

Sarah C.

Other

 

 

Item

Description

Lab Member

Ordering Chemicals, Supplies, 
Plasmids, RNAi, Equipment

 

Barbara and Sarah T.

Lab Website

Keep content up to date.

Sarah T. and Paul

Liquid Nitrogen/Gasses

Maintain adequate supply for weekends.  Information regarding the maintenance of our LN2 stock can be found here.

Barbara

-80° Freezers

Clean, maintain, instruct users.

Barbara

X-ray Source, Kerckhoff Basement (B121)

This is a shared resource.  Information regarding the use of the equipment can be found here.

Shared Resource

Pipetman

Call in service requests for calibration and/or repairs.  Info regarding service can be found here.

Barbara and Sarah T.

Strain Collection

Information about freezing worms can be found here.

Barbara

Media Solutions and Plates

 

All Lab Members

Printer

Maintain and order supplies.  Information regarding printer maintenance can be found here.

Barbara and Sarah T.

Microscope Front and Back Room, K221

Organize area.

Shayda

Jellyfish

 

Ravi