Our laboratory is interested in the systems biology of nematodes. We seek to understand how the properties of an organism are encoded in its genome.

We seek to understand how are the properties of an organism – its development, physiology and behavior – are encoded in its genome. We primarily use molecular genetics of the nematode Caenorhabditis elegans but also apply proteomics, computational biology, biochemistry, cell biology, imaging, behavioral analysis, optogenetics, synthetic biology to not only C. elegans but also parasitic nematodes, budding yeast, mice, jellyfish, fungi, and human cells as needed.

Specific areas of current interest are:

  • How does the 302-neuron worm brain assess the future environment based on computation of sensory and physiological inputs?
  • Does the male worm have a neural representation of its mating partner?
  • How can we best predict gene expression from DNA sequence?
  • What are the functional consequences of de novo genetic variants found in individual with autism spectrum disorder?
  • What changes in the nervous system occur during sleep?
  • How do cells migrate accurately during development?

Our major strategy is to perturb the activity of one or more genes and measure how cells or animals misbehave to infer gene function and genetic pathways. We measure gene expression by RNA-seq and transgenic reporters; we measure behavior using automated systems and optogenetics. We focus on intercellular signals and their transduction by the responding cell into transcriptional outputs. Many of the genes we have identified are the nematode counterparts of human genes, and we are leveraging C. elegansmolecular genetics to study human genes with no actionable information and of the consequence of variants found in human individuals, including those with autism spectrum disorder. Nematodes are major health and agricultural problems, so we focus on parasite relevant biology such as dauer/infective juvenile development, using genomics and molecular genetics.

Lab News

Our latest publication is now available on microPublication.org. Click here to read all about "Conserved missense variant in ALDH1A3 ortholog impairs fecundity in C. elegans". For a more in depth look at this article, check this out.

Our newest publication, "Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration" is availalbe online now. Click here for the article.

Now out in Communications Biology, "Autonomous adaptive data acquisition for scanning hyperspectral imaging". Check out the article here.

The Tianqiao and Chrissy Chen Institute for Neuroscience at Caltech is accepting applications for the BrainWAVE fellowship program. This program provides support for non-Caltech undergraduates, who are underrepresented in STEM and intent on pursuing a Ph.D. in neuroscience, to conduct a 10-week summer research project at Caltech. Fellows will receive $6,620, plus travel to and from Caltech, and $2,000 towards campus housing. Other program highlights include: faculty mentorship, graduate school prep workshops, research seminars, networking events, communication workshops, and social activities. Application deadline is January 9, 2021. Undergraduates interested in the 2021 BrainWAVE program are welcome to contact Paul Sternberg. For more details about the program, click here.

Our latest publication, The Gene Ontology resource: enriching a Gold mine is available here.

The paper, Modular metabolite assembly in C. elegans depends on carboxylesterases and formation of lysosome-related organelles has recently come out in eLife. Find the article here.

We now have a table of available cGAL Drive and Effector strains. Click here to check it out.

An exciting article about our newest Molecular Cell paper is currently available here.

Our peer-reviewed, single-result publication platform microPublication Biology is now included in PubMedCentral and indexed in PubMed. The Caltech Library is the publisher. This is the first biology journal designed to help get information into knowledge such as WormBase, ZFIN, SGD, FlyBase, and TAIR.

Undergraduate Student Sophie Walton has been awarded a Hertz Fellowship. Click here to read all about her amazing accomplishment.

Worm moving
  1. Kato M, Kolotuev I, Cunha A, Gharib S, Sternberg PW. (2021) Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration. PNAS. [epub ahead of print]. doi: 10.1073/pnas.1904338118.

  1. Wong WR, Maher S, Oh JY, Brugman KI, Gharib S, Sternberg PW. (2021). Conserved missense variant in ALDH1A3 ortholog impairs fecundity in C. elegans. microPublication Biology. 10.17912/micropub.biology.000357.