cGAL Project
Credit: Stephanie Nava, Wilber Palma
Credit: Stephanie Nava, Wilber Palma
Credit: Stephanie Nava, Wilber Palma
Credit: Stephanie Nava, Wilber Palma
Credit: Stephanie Nava, Wilber Palma
We are slowly expandibg the GAL4-UAS system adapted for C. elegans, cGAL and split cGAL, to develop community-wide reagents. Our goal is to create drivers that access all 302 hermaphrodite neurons and all male-specific neurons. The driver strains are created by selecting a single promoter, cGAL, or two intersecting promoters, split cGAL, expressed in the neuron to target specificity. Researchers can then cross the drivers with UAS effector strains, to produce progeny that will express the desired neuron/cell-specific gene expression. Our list of effectors contains inhibitors, reporter genes, neurotoxins, and others to facilitate future exploration.
As of now, we have developed drivers that can access various types of the 302 hermaphroditic neurons, such as sensory (52% completed), motor, interneurons, and polymodal neurons. We have also started the endeavor of creating drivers for male-specific neurons. Below we have listed a table of available drivers. The table includes the neuron name, which promoter/s were used, and driver strain name(s). The effector table includes the gene, its intended use, and effector strain name(s).
Strains are available upon request, for correspondence, you can email Paul orWilber
cGAL Drivers as of 9/22/23
| Neuron/Cell type | Promoter(s) | Description | PS# |
|---|---|---|---|
| ADE | dat-1 | ||
| ADF | srh-142 | Coelomocyte GFP ectopic expression | PS8461 available at CGC |
| ADL | srh-220 | Very weak expression | PS8091 (syEx) PS8462 available at CGC |
| ADL | sre-1 | PS9017 PS9018 PS9019 PS9020 PS9021 PS9035 available at CGC | |
| AFD | gcy-8 | PS8566 PS8567 PS8642 available at CGC | |
| AIM | pdf-1, mbr-1 | Additional neurons (possibly PVP) | PS8843 available at CGC |
| AIY | ttx-3 |
PS8290 available at CGC PS8291 PS8405 |
|
| ALA | flp-24 | PS8088 PS8089 PS7973 available at CGC | |
| ALM, PLM, AVM, PVM | mec-17 | PS8052 (syEx) PS8373 available at CGC PS8320 PS8323 | |
| ALN, PLN | nlp-20 | Short process length for both ALN and PLN | PS9665 (syEx) available at CGC PS9676 available at CGC PS9677 |
| ALN, PLN | T09B9.5 | Additional neurons (ventral cord neurons) | PS9666 (syEx) PS9675 available at CGC |
| ALN, PLN | C50F7.5 | Additional neurons (ventral cord neurons) | PS9895 PS9896 PS9897 PS9898 |
| AQR/PQR, URX | gcy-32 | Coelomocyte GFP ectopic expression | PS8844 available at CGC PS8845 |
| ASE | gcy-17 | PS8652 PS8563 available at CGC PS8654 | |
| ASER | gcy-5 | PS8650 available at CGC | |
| ASG | Y41C4A.6 | PS9028 PS9029 PS9030 available at CGC | |
| ASH | srj-26 | Additional neurons (ventral nerve cord neuron and a tail neuron) | PS9439 PS9545 PS9547 available at CGC |
| ASH | nlp-76 | Additional neurons (tail neuron) | PS9440 PS9549 PS9550 available at CGC |
| ASI | gpa-4 | Coelomocyte GFP ectopic expression | PS8658 PS9034 available at CGC |
| ASJ | trx-1 | PS8768 PS8772 PS8769 available at CGC | |
| ASJ | pps-1 | Minor fluorescence seen in non-neuronal tissue | PS9438 PS9542 available at CGC PS9543 |
| ASJ | F47D2.11 | PS9441 available at CGC PS9544 | |
| ASK | sra-9 | PS7789 PS7828 PS7829 available at CGC | |
| ASK | srd-36 |
PS9670 (syEx) PS9678 PS9893 available at CGC PS9894 |
|
| AVG | nmr-1, flp-7 | PS8128 (syEx) PS8287 PS8409 available at CGC PS8410 PS8411 PS8494 | |
| AVH | hlh-34 | PS8087 (syEx) PS8132 available at CGC PS8133 | |
| AVK | flp-1 | PS8458 PS8288 available at CGC PS8289 | |
| AWA | odr-10 | PS8292 PS8293 available at CGC PS8297 | |
| AWB | str-1 | General body GFP, coelomocyte GFP ectopic expression | PS8565 available at CGC PS8564 PS8649 |
| AWC | str-2 | General body GFP also present | PS8496 available at CGC PS8497 |
| AWC-OFF | srt-47 | PS9435 available at CGC PS9535 | |
| AWC-ON | srt-28 | PS9445 available at CGC | |
| BAG | gcy-33 | PS8562 available at CGC PS8563 | |
| Body muscle | myo-3 | PS6963 available at CGC | |
| CAN | pks-1 | PS9031 available at CGC PS9032 | |
| CEP | F35D11.1, F09E10.7 | PS9443 available at CGC PS9565 | |
| Cholinergic neurons | unc-17 | PS7155 available at CGC | |
| DD, VD | ttr-39 | PS9230 PS9231 PS9232 available at CGC PS9242 PS9243 | |
| Dopaminergic (CEP, ADE, PDE) | dat-1 | PS9663 (syEx) available at CGC PS9569 | |
| DVA | nlp-12 |
PS8022 (syEx) PS8014 (syEx) PS8415 PS8416 available at CGC PS8417 PS8418 |
|
| DVC | ceh-63 |
PS8129 PS8131 available at CGC |
|
| FLP, PVD | Y48G110A.6, F58F6.6 |
PS9673 available at CGC PS10078 PS10079 PS10080 PS10081 |
|
| GABAergic neurons | unc-47 | PS7166 PS7160 available at CGC PS7167 | |
| Gland cell | inx-11, flp-20 | PS8453 available at CGC PS8454 | |
| Glutamatergic neuron | eat-4 | PS7139 PS7140 PS7141 PS7142 PS7143 | |
| Heat shock | hsp.16.41 |
PS7169 PS7170 PS7171 PS7172 PS7173 |
|
| Hypodermis | dpy-7 |
PS10084 (syEx) PS10085 (syEx) |
|
| I1 | zag-1, nlp-3 | ||
| IL1 | aqp-6 | PS9240 PS9241 PS9235 available at CGC | |
| IL2 | klp-6 | PS8872 available at CGC PS8873 PS8874 | |
| Intestine | nlp-40 |
PS6916 available at CGC PS6933 available at CGC PS6934 available at CGC PS6935 available at CGC |
|
| M3 | flp-13, mnm-2 | ||
| MC | unc-17, ceh-19 |
PS7521 available at CGC PS7522 PS7523 |
|
| MCM | pdf-1, grl-2 |
PS8661 available at CGC PS8842 |
|
| NSM | tph-1, pdf-1 | PS8660 available at CGC | |
| OLL | eat4prom9, seb-3 |
PS9668 (syEx) available at CGC PS9901 PS9902 PS10076 PS10077 |
|
| OLQ | ocr-4 | PS9551 available at CGC | |
| Pan-neuronal | rab-3 |
PS6961 available at CGC PS6962 PS6963 available at CGC |
|
| PHA | srg-13 | PS8849 available at CGC PS8850 PS8851 | |
| PHA, PHB | srb-6, osm-10 | PS8846 available at CGC PS8847 PS8848 | |
| Pharyngeal muscle | myo-2 |
PS6844 available at CGC PS7154 available at CGC |
|
| PHC | F58F6.6 | Additional neurons (ADF and phasmid) | PS9534 available at CGC |
| PHC | capa-1, F58F6.6 | PS9899 PS9900 | |
| PHC | kcnl-4, F58F6.6 | PS9672 (syEx) available at CGC PS9557 | |
| PVC | nmr-1, flp-20 | Weak expression, present in half of the animals |
PS8126 (syEx) PS8282 available at CGC PS8283 |
| PQR | gcy-32 | ||
| PVD | Y48G10A.6, kcnl-4 | ||
| PVQ | nlp-17 |
PS8839 available at CGC PS8840 PS8841 |
|
| RIA | glr-3 |
PS8281 (syEx) PS8279 available at CGC PS8280 |
|
| RIC | tbh-1 | PS9229 available at CGC PS9236 PS9237 PS9238 PS9239 | |
| rig-5 | Better images needed to identify | PS8643 available at CGC | |
| RMG | nlp-56 |
PS8834 available at CGC PS8835 PS8836 PS8837 |
|
| URB | nlp-48 | PS9560 PSP9561 | |
| URX | gcy-32 | PS8648 available at CGC | |
| URY | arrd-16 | Additional neurons (DVA, DVC, and interneuron) |
PS9664 available at CGC PS10082 PS10083 |
Effector Strains as of 9/22/23
| Transgene | Description | |
|---|---|---|
| UAS::-PKA(DN)-SL2-GFP | Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons. | PS8124 PS8125 |
| UAS::-FEM-3 | Gene involved in sex determination, induces masculinization | PS8509 |
| UAS::-TRA-2 (IC) | Gene involved in sex determination, induces feminization | PS8408 |
| UAS::-Lin-3C | Epidermal growth factor | PS8278 |
| UAS::-GtACR2-SL2-GFP | Natural light-gated anion channels, inhibition with blue light | PS8284 PS8285 PS8286 |
| UAS::-destabilized-YFP | PS8406 PS8407 | |
| UAS::-wrmScarlet | C. elegans optimized version of mScarlet | PS8459 PS8460 |
| UAS::-sbt-1 | Tissue specific sbt-1 expression is sufficient to rescue dauer entry defect in sbt-1 mutant | PS7901 (syEx) PS8420 PS8421 PS8422 |
| UAS::-TeTX-SL2-mKate | In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins. | PS8412 PS8413 PS8414 |
| UAS::-TeTX-SL2-GFP | In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins. | PS9432 PS9433 PS9434 |
| UAS::-GCaMP7s-SL2-mKate | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Latest GCaMP, improved SNR, slow kinetics | PS8419 |
| UAS::-GCaMP7f-SL2-mKate | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Latest GCaMP, improved SNR, fast kinetics | PS8423 PS8424 |
| UAS::-GCaMP7b-SL2-mKate | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Latest GCaMP, improved SNR, higher baseline fluorescence | PS8473 PS8495 |
| UAS::-lmp1-venus | Lysosomal associated membrane protein | PS8474 PS8475 PS8476 PS8477 |
| UAS::-iC1-c2-TS-EYFP | Chloride-conducting channelrhodopsin | PS8466 PS8467 |
| UAS::-rpl-22HA-SL2-mKate | For tissue specific RNA-seq | PS8463 PS8464 PS8465 |
| UAS::-act2-venus | Localizes to actin filament and cell cortex |
PS8555 PS8556, PS8557 |
| UAS::-nCRE-SL2-GFP | Nucleolus-transport-enhanced Cre protein. Recombination-mediated gene expression knockout. |
PS8469 PS8470 PS8471 PS8472 |
| UAS::-ChR2(C128S)-EYFP | Stable step function ChR2 variant. Activated by blue light, terminated by green light. |
PS8498 PS8499 PS8500 |
| UAS::-SwiCHR-TS-EYFP | Step-Waveform Inhibitory Channelrhodopsin |
PS8508 PS8510 |
| UAS::-hChR2 (C128S, D156A)-EYFP | Stabilized step function Opsins ChR2 variant |
PS8503 PS8504 PS8507 |
| UAS::-miniSOG-130L-VAMP2-mCherry | Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production. |
PS8505 PS8506 |
| UAS::-myri-mCherry | Fluorophore used to target and label cell membrane. Leaky RFP (seen in tails) |
PS8501 PS8502 |
| UAS::PTX-SL2-GFP | Inactivation of G protein subunits through Pertussis toxin (PTX) | *plasmid available * |
| UAS::VSFP-Butterfly-1-2 | Voltage-sensitive fluorescent protein |
PS8552 PS8553 PS8554 |
| UAS::miniSOG-103L::mCherry | Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production. | PS8558 |
| UAS::GCaMP6s::SL2::mKate2 | In vivo calcium indicator. Presence of calcium (Ca2+) increase reporter signal intensity. |
PS7203 PS7205 PS7206 PS7207 PS7208 |
| UAS::hChR2(H134R)::YFP | Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron depolarization through brief pulses of blue light. |
PS7043 PS7044 PS7045 |
| UAS::HisCl1::SL2::GFP | In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 trasngene are inhibited after addition of histamine. |
PS7199 PS7107 PS7108 |
| UAS::TeTx | In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins. |
PS7200 PS7201 PS7202 |
| UAS::ICE | Human caspase 1 (ICE) directed cell death. |
PS7192 PS7193 PS7194 PS7195 PS7195 |
| UAS::Chrimson-GFP | Red light-activated channelrhodopsin | PS7900 PS8023 PS8024 |
| UAS::Chrimson-tdTomato | Red light-activated channelrhodopsin | PS9436 PS9538 |