cGAL Project
Credit: Stephanie Nava, Wilber Palma
Credit: Stephanie Nava, Wilber Palma
Credit: Stephanie Nava, Wilber Palma
Credit: Stephanie Nava, Wilber Palma
Credit: Stephanie Nava, Wilber Palma
We wish to expand the GAL4-UAS system adapted for C. elegans, cGAL and split cGAL, to develop community-wide reagents. Our goal is to create drivers that access all 302 neurons, as well as male-specific neurons. The driver strains are created by selecting a single promoter, cGAL, or two intersecting promoters, split cGAL, expressed in the neuron to target specificity. Researchers can then cross the drivers with UAS effector strains, to produce progeny that will express the desired neuron/cell-specific gene expression. Our list of effectors contains inhibitors, reporter genes, neurotoxins, and others to facilitate future exploration.
As of now, we have developed drivers that can access various types of the 302 hermaphroditic neurons, such as sensory (52% completed), motor, interneurons, and polymodal neurons. We have also started the endeavor of creating drivers for male-specific neurons. Below we have listed a table of available drivers. The table includes the neuron name, which promoter/s were used, and driver strain name(s). The effector table includes the gene, its intended use, and effector strain name(s).
Strains are available upon request, for correspondence, you can email Paul, Wilber, or Stephanie.
cGAL Drivers as of 3/23/22
| Neuron/Cell type | Promoter(s) | Description | PS# |
|---|---|---|---|
| ADF | srh-142 | Coel::GFP ectopic expression | PS8461 |
| ADL | srh-220 | Very weak expression |
PS8091 (syEx) PS8462 |
| ADL | sre-1 | PS9017, PS9018, PS9019, PS9020, PS9021 | |
| AFD | gcy-8 |
PS8566 PS8567 PS8642 |
|
| AIM | pdf-1, mbr-1 | Additional neurons (possibly PVP) | PS8843 |
| AIY | ttx-3 |
PS8290 PS8291 PS8405 |
|
| ALA | flp-24 |
PS8088 PS8089 |
|
| ALM, PLM, AVM, PVM | mec-17 |
PS8052 (syEx) PS8373 PS8320 PS8323 |
|
| AQR/RQR, URX | gcy-32 | ectopic coel in gfp | PS8844, PS8845 |
| ASE | gcy-17 | PS8652, PS8653, PS8654 | |
| ASEL | gcy-5 | PS8650 | |
| ASG | Y41C4A.6 | PS9028, PS9029, PS9030 | |
| ASH | srj-26 | gfp in a ventral nerve, cord neuron and a tail neuron | PS9439, PS9545, PS9547 |
| ASH | nlp-76 | gfp in tail neuron | PS9440, PS9549, PS9550 |
| ASI | gpa-4 | Coel::GFP ectopic expression | PS8658 |
| ASJ | trx-1 | PS8768, PS8772, PS8769 | |
| ASJ | pps-1 | Minor fluorescence seen in non-neuronal tissue | PS9438, PS9542, PS9543 |
| ASJ | F47D2.11 | PS9441, PS9544 | |
| ASK | sra-9 |
PS7789 PS7828 |
|
| AVG | nmr-1, flp-7 |
PS8128 (syEx) PS8287 (syEx) PS8409 PS8410 PS8411 PS8494 |
|
| AVH | hlh-34 |
PS8087 (syEx) PS8132 PS8133 |
|
| AVK | flp-1 |
PS8458 PS8288 PS8289 |
|
| AWA | odr-10 |
PS8292 PS8293 PS8297 |
|
| AWB | str-1 | General body GFP also present, coel::gfp |
PS8565 PS8564 PS8649 |
| AWC | str-2 | General body GFP also present |
PS8496 PS8497 |
| AWC-OFF | srt-28 | PS9435, PS9535 | |
| AWC-ON | srt-47 | PS9445 | |
| BAG | gcy-33 |
PS8562 PS8563 |
|
| Body muscle | myo-3 | PS6936 | |
| CAN | pks-1 | PS9031, PS9032 | |
| CEP | F35D11.1, F09E10.7 | PS9443, PS9565 | |
| Cholinergic Neuron | unc-17 | PS7155 | |
| DD/VD | ttr-39 | PS9230, PS9231, PS9232, PS9242, PS9243 | |
| Dopaminergic (CEP, ADE, PDE) | dat-1 | SyEX1708 | |
| DVA | nlp-12 |
PS8022 (syEx) PS8014 (syEx) PS8415 PS8416 PS8417 PS8418 |
|
| DVC | ceh-63 |
PS8129 PS8131 |
|
| GABAergic neuron | unc-47 |
PS7166 PS7160 PS7167 |
|
| Gland cell | inx-11, flp-20 |
PS8453 PS8454 |
|
| Glutamatergic neuron | PS7139, PS7140, PS7141, PS7142, PS7143 | ||
| Heat shock | hsp.16.41 |
PS7169 PS7170 PS7171 PS7172 PS7173 |
|
| I1 | zag-1, nlp-3 | ||
| IL1 | aqp-6 | PS9240, PS9241 | |
| IL2 | klp-6 | PS8872, PS8873, PS8874 | |
| Intestine | nlp-40 |
PS6916 PS6933 PS6934 PS6935 |
|
| M3 | flp-13, mnm-2 | ||
| MC | unc-17, ceh-19 |
PS7521 PS7522 PS7523 |
|
| MCM | pdf-1, grl-2 |
PS8661 PS8842 |
|
| NSM | tph-1, pdf-1 | PS8660 | |
| OLL | eat4prom11, flp-11 | Needs to be reconstructed | |
| OLQ | ocr-4 | PS9551 | |
| Pan-neuronal | rab-3 |
PS6961 PS6962 PS6963 |
|
| PHA | Srg-13 | PS8849, PS8850, PS8851 | |
| PHA/PHB | srb-6, osm-10 | PS8846, PS8847, PS8848 | |
| Pharyngeal muscle | myo-2 |
PS6844 PS7154 |
|
| PHC | F58F6.6 | GFP in ADF and in another plasmid | PS9534, PS9567, PS9568, PS9569 |
| PVC | nmr-1, flp-20 | Weak expression, present in half of the animals. |
PS8126 (syEx) PS8282 PS8283 |
| PVQ | nlp-17 |
PS8839 PS8840 PS8841 |
|
| RIA | glr-3 |
PS8281 (syEx) PS8279 PS8280 |
|
| RIC | tbh-1 | PS9229, PS9236, PS9237, PS9238, PS9239 | |
| RMD? | rig-5 | Better images needed to identify | PS8643 |
| RMG | nlp-56 |
PS8834 PS8835 PS8836 PS8837 |
|
| URB | nlp-48 | PS9560, PSP9561 | |
| URX | flp-8 | GFP seen in AUA | PS8648 |
| URY | arrd-16 | GFP seen in DVA, DVC, and interneuron | syEX1709 |
Effector Strains as of 7/30/2020
| Transgene | Description | |
|---|---|---|
| UAS::-PKA(DN)-SL2-GFP | Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons. | PS8124 PS8125 |
| UAS::-FEM-3 | Gene involved in sex determination, induces masculinization | PS8509 |
| UAS::-TRA-2 (IC) | Gene involved in sex determination, induces feminization | PS8408 |
| UAS::-Lin-3C | Epidermal growth factor | PS8278 |
| UAS::-GtACR2-SL2-GFP | Natural light-gated anion channels, inhibition with blue light | PS8284 PS8285 PS8286 |
| UAS::-destabilized-YFP | PS8406 PS8407 | |
| UAS::-wrmScarlet | C. elegans optimized version of mScarlet | PS8459 PS8460 |
| UAS::-sbt-1 | Tissue specific sbt-1 expression is sufficient to rescue dauer entry defect in sbt-1 mutant | PS7901 (syEx) PS8420 PS8421 PS8422 |
| UAS::-TeTX-SL2-mKate | In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins. | PS8412 PS8413 PS8414 |
| UAS::-TeTX-SL2-GFP | In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins. | PS9432 PS9433 PS9434 |
| UAS::-GCaMP7s-SL2-mKate | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Latest GCaMP, improved SNR, slow kinetics | PS8419 |
| UAS::-GCaMP7f-SL2-mKate | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Latest GCaMP, improved SNR, fast kinetics | PS8423 PS8424 |
| UAS::-GCaMP7b-SL2-mKate | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Latest GCaMP, improved SNR, higher baseline fluorescence | PS8473 PS8495 |
| UAS::-lmp1-venus | Lysosomal associated membrane protein | PS8474 PS8475 PS8476 PS8477 |
| UAS::-iC1-c2-TS-EYFP | Chloride-conducting channelrhodopsin | PS8466 PS8467 |
| UAS::-rpl-22HA-SL2-mKate | For tissue specific RNA-seq | PS8463 PS8464 PS8465 |
| UAS::-act2-venus | Localizes to actin filament and cell cortex |
PS8555 PS8556, PS8557 |
| UAS::-nCRE-SL2-GFP | Nucleolus-transport-enhanced Cre protein. Recombination-mediated gene expression knockout. |
PS8469 PS8470 PS8471 PS8472 |
| UAS::-ChR2(C128S)-EYFP | Stable step function ChR2 variant. Activated by blue light, terminated by green light. |
PS8498 PS8499 PS8500 |
| UAS::-SwiCHR-TS-EYFP | Step-Waveform Inhibitory Channelrhodopsin |
PS8508 PS8510 |
| UAS::-hChR2 (C128S, D156A)-EYFP | Stabilized step function Opsins ChR2 variant |
PS8503 PS8504 PS8507 |
| UAS::-miniSOG-130L-VAMP2-mCherry | Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production. |
PS8505 PS8506 |
| UAS::-myri-mCherry | Fluorophore used to target and label cell membrane. Leaky RFP (seen in tails) |
PS8501 PS8502 |
| UAS::PTX-SL2-GFP | Inactivation of G protein subunits through Pertussis toxin (PTX) | *plasmid available * |
| UAS::VSFP-Butterfly-1-2 | Voltage-sensitive fluorescent protein |
PS8552 PS8553 PS8554 |
| UAS::miniSOG-103L::mCherry | Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production. | PS8558 |
| UAS::GCaMP6s::SL2::mKate2 | In vivo calcium indicator. Presence of calcium (Ca2+) increase reporter signal intensity. |
PS7203 PS7205 PS7206 PS7207 PS7208 |
| UAS::hChR2(H134R)::YFP | Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron depolarization through brief pulses of blue light. |
PS7043 PS7044 PS7045 |
| UAS::HisCl1::SL2::GFP | In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 trasngene are inhibited after addition of histamine. |
PS7199 PS7107 PS7108 |
| UAS::TeTx | In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins. |
PS7200 PS7201 PS7202 |
| UAS::ICE | Human caspase 1 (ICE) directed cell death. |
PS7192 PS7193 PS7194 PS7195 PS7195 |
| UAS::Chrimson-GFP | Red light-activated channelrhodopsin | PS7900 PS8023 PS8024 |
| UAS::Chrimson-tdTomato | Red light-activated channelrhodopsin | PS9436 PS9538 |